Composite

Part:BBa_K1111009

Designed by: Luisa Spisak, Marcelle Isaline Arrigo   Group: iGEM13_EPF_Lausanne   (2013-09-19)
Revision as of 18:40, 1 October 2013 by Miarrigo (Talk | contribs)

Gelatinase under pBAD/araC arabinose inducible promoter

This construct is composed of gelatinase E's (gelE) coding sequence(CDS)inserted between the pBAD/araC promoter and superfolder GFP of part BBa_I746908 from Cambridge 2007. A linker is also present to join gelE and GFP correctly and to allow proper folding. Upon arabinose addition in the culture medium of bacteria transformed with this plasmid, gelE-GFP will be expressed and secreted

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3101
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal PstI site found at 3101
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2634
    Illegal BglII site found at 2685
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3028
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3101
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3101
    Illegal NgoMIV site found at 2330
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2703
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1517
    Illegal BsaI.rc site found at 2234
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 3169


[edit]
Categories
Parameters
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