Coding
TAL
Part:BBa_K1033000:Design
Designed by: Karl Holdar, Hampus Elofsson, Emil Marklund, Kristoffer Lundmark, Marcus Hong, Lovisa Pettersson, Ken Braech-Andersen, Theodor Löwe Group: iGEM13_Uppsala (2013-08-15)
Tyrosine ammonia-lyase (TAL) with RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 45
Illegal NgoMIV site found at 877
Illegal AgeI site found at 140
Illegal AgeI site found at 306 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1335
Design Notes
Earlier in iGEM eukaryot TAL has been used, however we used bacterial TAL for better compatibility with bacteria.
Source
Rhodobacter sphaeroides