Part:BBa_K1189024
Light chain human ferritin
This light ferritin chain comes from humans. This part along with heavy ferritin (BBa_K1189025), form the ferritin nanoparticle, an iron-storage particle made up of 24 subunits. The formed nanoparticle is a robust, remaining stable at extreme pHs and temperatures. The light ferritin purportedly contributes to nucleation to initiate iron core formation in ferritin molecules.
Figure 1. Ribbon visualization of a fully assembled ferritin protein.
This nanoparticle can also be used as a reporter when the iron core is modified with potassium ferrocyanide to form Prussian Blue. The Prussian Blue ferritin can then act as a peroxidase mimic, similar to horseradish peroxidase, resulting in colour changes in the presence of hydrogen peroxide, and TMB or ABTS.
This part was primarily used to construct BBa_K1189020 in order to allow us to express and purify the protein.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 391
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