Reporter

Part:BBa_K1182000:Design

Designed by: Muhammad Hanifi   Group: iGEM13_UI-Indonesia   (2013-09-16)
Revision as of 19:41, 30 September 2013 by Hanifi UI (Talk | contribs) (→‎References)


Split β-galactosidase - ω fragment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

The part is fragment of E.coli LacZ gene, acquired by PCR.

References

Stains, C.I., et al. (2010). A General Approach for Receptor and Antibody-Targeted Detection of Native Proteins utilizing Split-Luciferase Reassembly. ACS Chem Biol. 5(10): 943–952. doi:10.1021/cb100143m.

Broome, A.M., et al. (2010). Expanding the utility of Beta-galactosidase complementation: pieceby piece. Mol Pharm. 7(1): 60–74. doi:10.1021/mp900188e.