Device

Part:BBa_K1150025

Designed by: Freiburg 2013   Group: iGEM13_Freiburg   (2013-09-17)
Revision as of 13:17, 18 September 2013 by ChristophGabi (Talk | contribs) (→‎Introduction)

uniCAS Red Light Switch Part I - Stimulator

Introduction

Our construct contains the standardized version of Cas9 linked to PIF6. This device is one of two parts that you need to enable red-light induced gene targeting. Cotransfection of a PhyB+effector device, and target RNA allows you to recruit any desired effector to your desired Gene locus. Illumination with red light (660nm) will start the interaction and illumination with far red light (740nm) will stop the interaction. The Cas9-version used here is human codon optimized and the mutated version, unable to cleave DNA. PIF6 is human codon-ptimized.

References:

Muller, K.; Engesser, R.; Metzger, S.; Schulz, S.; Kampf, M. M.; Busacker, M. et al. ((Nucleid Acid Research; 2013): A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BglII site found at 900
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/auniCAS Red Light Switch Part I - Stimulator