Not Released
Sample In stock
Experience: Works
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Composite

Part:BBa_K1149050

Designed by: Margarita Kopniczky   Group: iGEM13_Imperial_College   (2013-09-24)
Revision as of 13:25, 27 September 2013 by Margarita K (Talk | contribs)

Bdh2 (intracellular)

This part expresses Bdh2 3HB dehydrogenase enzyme intracellularly. There is a GFP in operon with the enzyme which can be used to monitor expression levels. The promoter is arabinose inducible pBAD.

http://www.igem.org/wiki/images/f/f0/Reaction_bdh.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 181
    Illegal AgeI site found at 1126
  • 1000
    COMPATIBLE WITH RFC[1000]


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