Device
Part:BBa_K1065101
Designed by: Caterina Marchioretti Group: iGEM13_UNITN-Trento (2013-06-25)
AraCpBAD + BSMT1
This is the part BBa_K1065100 under the control of the inducibile promoter AraCpBAD. This promoter allows to obtain the production of Methyl Salicylate starting from Salicyclic Acid when 5 mM of Arabinose was added.
Usage and Biology
BSMT1 catalyzes the conversion of salicylic acid to methyl salicylate, which has a wintergreen smell.
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The arabinose inducible promoter BBa_K731201 was fully characterized by iGEM UniTN Trento 2012.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 1893 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1434
Illegal SapI site found at 961
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Categories
Parameters
None |