RNA
Part:BBa_K1072010
Designed by: Junqi kuang, Xihao liao and Jianhui gong Group: iGEM13_SCUT (2013-09-09)
Revision as of 06:42, 22 September 2013 by Junjiezhang (Talk | contribs)
Odr-10(diacetyl)GPCR with optimized codon
Odr-10(diacetyl)GPCR encodes a diacetyl-sensing G-protein coupled receptor, originating from the C. elegans.
If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein. pathway is composed of Gpa1 (Gα), Ste4 (Gβ) and Ste18 (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will links a reproter, such as GFP, His3 or LacZ.
Figure 1. a diagram of Odr-10, 7-transmembrane receptors.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/receptor
//chassis/eukaryote/yeast
//function/cellsignalling
//chassis/eukaryote/yeast
//function/cellsignalling
chassis | yeast |
family | GPCR |
function | diacetyl sensing GPCR |
ligands | diacetyl |
origin | C.elegans ODR-10 |
tag | FLAG and GFP |