Coding

Part:BBa_K1067002

Designed by: Kristian Davidsen and Helen Cook   Group: iGEM13_DTU-Denmark   (2013-09-19)
Revision as of 15:51, 28 September 2013 by KrDa (Talk | contribs)

Cytochrome A and X from Nitrosomonas europaea

Cytochrome A (c-554) and cytochrome X (Probable tetraheme cytochrome c-type) both from Nitrosomonas europaea.

The two cytochromes are involved in the process of transferring electrons from HAO to AMO in the following reactions:

HAO reaction, 2 electrons gained:

NH2OH + H2O + 2 cycAXox → NO2- + 2 cycAXred + 5 H+

AMO reaction 2 electrons used:

NH3 + O2 + cycAXred + 2 H+ → NH2OH + H2O + cycAXox


Usage and Biology

When highly expressed in E.coli the cells turn dark red. If grown on media with supplementation of d-aminolevulinic acid (ALA) it tends to get a darker color because of the extra heme incorporated.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal PstI site found at 51
    Illegal PstI site found at 490
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal PstI site found at 51
    Illegal PstI site found at 490
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal BglII site found at 448
    Illegal BamHI site found at 235
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal PstI site found at 51
    Illegal PstI site found at 490
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 686
    Illegal EcoRI site found at 838
    Illegal PstI site found at 51
    Illegal PstI site found at 490
    Illegal NgoMIV site found at 1140
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//function/biosynthesis
Parameters
protein