Part:BBa_K1073020:Experience

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Applications of BBa_K1073020

When aeBlue is expressed, the colonies on plates as well as the liquid culture become blue.
The iGEM Team Braunschweig 2013 used this device to distinguish two different strains.

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••••• iGEM Team Braunschweig 2013

The chromoprotein aeBlue exhibits a distinct blue color when expressed. In order to avoid absorption by the chomoproteins during OD measurement of a bacterial culture, the absorption and emission spectra were measured. An optimal wavelength for spectral optical density measurement was determined to be at 520nm according to these spectra. iGEM Team Braunschweig 2013: Absorption spectrum of chromoprotein aeBlue in a wavelength range of 400-800 nm. Absorption maximum of aeBlue: 595 nm iGEM Team Braunschweig 2013:Emission spectrum of chromoprotein aeBlue. This chromoprotein does not show any significant emission in a wavelength range between 500-800 nm and therefore cannot be detected by its fluorescence. Emission maximum of aeBlue: none (dark quencher) AeBlue is a so called dark quencher protein. When excited, this kind of chromophores relax back to the ground state without emitting energy through radiation and remain dark [Le Reste et al., 2012]. The optical density of cultures with cells expressing this protein was measured at a wavelength of 520 nm to avoid interference through absorption by aeblue.

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