Device
Part:BBa_K1051900
Designed by: Yang Zhou Group: iGEM13_Shenzhen_BGIC_ATCG (2013-09-25)
G1 phase magic with cell synchronization device and CRISPRi induced alternative splicing device.
clb5 +d-box +GFP + src1 intron + tom40 + cca + gal + dCas9 + cca + snr52 + sgRNA(Hub1) + sup4
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 6680
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 5088
Illegal AgeI site found at 1943
Illegal AgeI site found at 7092 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5234
Illegal BsaI site found at 6088
Illegal BsaI.rc site found at 1396
Illegal BsaI.rc site found at 3759
Illegal SapI site found at 1745
Illegal SapI.rc site found at 7807
[edit]
Categories
Parameters
n/a | G1 phase magic with cell synchronization device and CRISPRi induced alternative splicing device. |