Composite

Part:BBa_K1065307:Design

Designed by: Caterina Marchioretti   Group: iGEM13_UNITN-Trento   (2013-09-15)
Revision as of 10:49, 28 September 2013 by Cate (Talk | contribs) (→‎Source)


J23100+YF1+FixJ+terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


Design Notes

This part was built following the standard assembly protocol using BBa_K1065306 and BBa_B0015.

Source

This part has been built using:
BBa_K1065307 produces YF1 and FixJ under the control of a strong promoter of the Anderson family.
BBa_B0015 the terminator.

References

1. Andreas Möglich, Rebecca A. Ayers, Keith Moffat (2009). Design and Signaling Mechanism of Light-Regulated Histidine Kinases. Journal of Molecular Biology, 1433–1444.