Coding

Part:BBa_K1189009:Design

Designed by: Taylor Remondini   Group: iGEM13_Calgary   (2013-09-17)
Revision as of 05:28, 28 September 2013 by Rai (Talk | contribs)

Beta-lactamase with His Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note: The Bsa1 cut site has not been removed.


TCT: Original serine - mutated to TCC: serine Mutagenesis Primers F: 5’GGAGCCGGTGAGCGTGGGTCCCGCGGTATCATTGCAGC 3’ R: 5’GCTGCAATGATACCGCGGGACCCACGCTCACCGGCTCC 3’