Coding
Part:BBa_K1164002
Designed by: Wilson Lam, Dylan Siriwardena Group: iGEM13_uOttawa (2013-09-16)
LacI repressor tagged with yBFP
This is the LacI repressor from E.coli tagged with yeast codon optimized BFP. LacI will bind with high specificity to the the operator lacO and is inhibited in the presence of IPTG. BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm. It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.
This is an updated version of BBa_K642001 that is functional in S. cerevisiae.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1044
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 595
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |