Device

Part:BBa_K1164008

Designed by: Wilson Lam, Dylan Siriwardena, Lloyd Mai, Yara Abou-Hamde   Group: iGEM13_uOttawa   (2013-09-16)
Revision as of 20:51, 16 September 2013 by IMaginAZN (Talk | contribs)

pTRELX driving yeGFP with tPGK1 terminator

This device consists of a modified version of the native Gal1 promoter found in S.cerevisiae driving production of yeast codon optimized GFP. The four gal4 binding domains present in the native Gal1 promoter have been replaced by four tetO sites. This promoter may be activated by regulatory activators that are able to bind to tetO. In addition, a lacO operator has been introduced downstream of the TATA box, allowing for repression of gene expression by lacI. Upon expression from the promoter, yEGFP will be produced.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 465
    Illegal BamHI site found at 553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 81
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1202


[edit]
Categories
Parameters
None