Part:BBa_K1092005:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Characterization of BBa_K1092005

1. Biobrick DNA length verification

To check the inserted biobrick DNA length, PCR was conducted (Figure 1).

The PCR product meets the target size (1542 bp), the DNA sequence was further verified by DNA sequencing.

2. Protein activity test by automatic kinetic spectrophotometric assay using o-phenylenediamine

Laccase is capable of oxidizing o-phenylenediamine (1,2-diaminobenzene) during the stopped-flow period. During oxidation, 2,3-diaminophenazin, that can be determined spectrophotometrically, will be produced. So the kinetic spectrophotometric method can be used to detect laccase activity (Huang et al., 1996).

User Reviews

UNIQ55d952c1867f1b5a-partinfo-00000000-QINU UNIQ55d952c1867f1b5a-partinfo-00000001-QINU