Regulatory

Part:BBa_K1087004:Experience

Designed by: Tong Xu   Group: iGEM13_SCU_China   (2013-09-12)
Revision as of 03:10, 28 September 2013 by Nianhui (Talk | contribs) (→‎User Reviews)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1087004

User Reviews

UNIQd0358625d3db9ab7-partinfo-00000000-QINU UNIQd0358625d3db9ab7-partinfo-00000001-QINU We assemblied BBa_K1087004 with mRFP to create BBa_K1087015, and we ligate BBa_K1087015 with RiboKey BBa_K145215 to create BBa_K1087021. Then, we transformed BBa_K1087015, BBa_K1087021 into E.coli DH5α,respectively. And we use Ptet+mRFP1(BBa_I13521) as positive control, irrelevant BBa_J61046 with no fluorescence gene as negative control. All of the constructs were expressed in high copy plasmid PSB1X3.

According to Data,the ribolock BBa_K1087004 works well, and its fluorescence intensity was only 2.6531% compared to positive control even at 19h, while that of lock & key (BBa_K1087021) was 91.7138% at 19h. The results suggest the key BBa_K145215 can hugely improve the expression level of the lock BBa_K1087004.