Coding
ATF1

Part:BBa_J45014

Designed by: André Green II   Group: iGEM06_MIT   (2006-07-31)
Revision as of 19:21, 31 July 2006 by Drephd (Talk | contribs)

alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)

This part is the ATF sequence BBa_45J006 with a single point mutation at base pair 1148 (T -> C). Alcohol acetyltransferase I (ATFI) encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols. It was obtained from S. cerevisiae.

Usage and Biology

We (MIT iGEM 2006) are currently experimenting with this part to determine its functional charateristics.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BamHI site found at 1426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526
    Illegal SapI.rc site found at 1534


[edit]
Categories
//function/biosynthesis/odorant
//function/odor
s
Parameters
ec_num2.3.1.84
keggsce:YOR377W
proteinATF1-1148 mutant
swissproP40353