Generator

Part:BBa_K1021033:Design

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-15)
Revision as of 18:28, 15 September 2013 by Registry (Talk | contribs)

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PT7+hph


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 391
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As this part was not designed for bacterial expression, it does not contain a bacterial RBS.


Source

The promoter comes from the T7 virus; hph comes from E. coli and was taken from the pUCATPH transformation vector.

References