Part:BBa_K1080010
YCF54
Long Descriptions here
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 108
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 270
Amino acid sequence
MAPAAASADK ATAAEYYALV CNAEWFFMDP QNESVAEQLR EKVRFFKEQN KERDFFIVPN
PKWLDAKFPE QAKQVKRPCV ALVSTDKMWI TFMKLRLDRV LKIDLKSMPA SEVLAAGEAL
PDFKPDGKWT APYARYTPGW WNVFLPNH </F>
References and documentation are available.
Please note the modified algorithm for extinction coefficient.
Number of amino acids: 148
Molecular weight: 17073.7
Theoretical pI: 8.50
Amino acid composition: Ala (A) 20 13.5% Arg (R) 7 4.7% Asn (N) 6 4.1% Asp (D) 9 6.1% Cys (C) 2 1.4% Gln (Q) 5 3.4% Glu (E) 10 6.8% Gly (G) 3 2.0% His (H) 1 0.7% Ile (I) 3 2.0% Leu (L) 11 7.4% Lys (K) 14 9.5% Met (M) 5 3.4% Phe (F) 10 6.8% Pro (P) 12 8.1% Ser (S) 5 3.4% Thr (T) 5 3.4% Trp (W) 6 4.1% Tyr (Y) 4 2.7% Val (V) 10 6.8% Pyl (O) 0 0.0% Sec (U) 0 0.0%
(B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 21
Atomic composition:
Carbon C 785 Hydrogen H 1190 Nitrogen N 202 Oxygen O 212 Sulfur S 7
Formula: C785H1190N202O212S7 Total number of atoms: 2396
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 39085 Abs 0.1% (=1 g/l) 2.289, assuming all pairs of Cys residues form cystines
Ext. coefficient 38960
Abs 0.1% (=1 g/l) 2.282, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is:
30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.64 This classifies the protein as stable.
Aliphatic index: 70.00
Grand average of hydropathicity (GRAVY): -0.385
Source
Chlamydomonas reinhardtii
References
None |