Regulatory

Part:BBa_K1026009:Design

Designed by: Hongyi WU   Group: iGEM13_SJTU-BioX-Shanghai   (2013-09-27)
Revision as of 01:14, 28 September 2013 by Wuhongyi (Talk | contribs) (→‎Design Notes)


A rationally designed promoter negative regulated by CcaR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 42
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We discovered a segment around the -35 region of BBa_J23107 that is almost identical to G-box, the binding region of CcaR on PcpcG2. So we put the G-box onto this exact region, between -35 region and -10 region of the original promoter. Therefore, we expect that activated CcaR will bind and hinder RNA polymerase binding, and thus prevents transcription initialization. File:Rationally Designed Green Promoter.png

Source

Synechocystissp. PCC 6803

References