Part:BBa_K1019003

mlrA

MlrA is a transcriptional regulator of the curli operon in an avian pathogenic E. coli strain and in S. enterica subsp. serovar Typhimurium. Its conserved sequence in E. coli K-12 substr. MG1655 is expected to upregulate csgD expression and thus increase biofilm formation.

For further information and extensive references please visit [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG12008 EcoCyc]

References

Brown, P. K., Dozois, C. M., Nickerson, C. A., Zuppardo, A., Terlonge, J., & Curtiss, R. (2001). MlrA, a novel regulator of curli (AgF) and extracellular matrix synthesis by Escherichia coli and Salmonella enterica serovar Typhimurium. Molecular microbiology, 41(2), 349-363.

Ogasawara, H., Yamamoto, K., & Ishihama, A. (2010). Regulatory role of MlrA in transcription activation of csgD, the master regulator of biofilm formation in Escherichia coli. FEMS microbiology letters, 312(2), 160-168.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 146
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]