Part:BBa_K1019001

CsgD: positive regulator of curlin genes

CsgD is a positive regulator of genes involved in curli adhesin protein assembly, transport, and structure. Curli are an important feature of biofilms and help bacteria adhere to both each other and their surrounding surfaces.

For detailed information and extensive references please visit [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=G6546 EcoCyc]

References

Hammar, M. R., Arnqvist, A., Bian, Z., Olsén, A., & Normark, S. (1995). Expression of two csg operons is required for production of fibronectin‐and Congo red‐binding curli polymers in Escherichia coli K‐12. Molecular microbiology, 18(4), 661-670.

Loferer, H., Hammar, M., & Normark, S. (1997). Availability of the fibre subunit CsgA and the nucleator protein CsgB during assembly of fibronectin‐binding curli is limited by the intracellular concentration of the novel lipoprotein CsgG. Molecular microbiology, 26(1), 11-23.

Brombacher, E., Dorel, C., Zehnder, A. J., & Landini, P. (2003). The curli biosynthesis regulator CsgD co-ordinates the expression of both positive and negative determinants for biofilm formation in Escherichia coli. Microbiology, 149(10), 2847-2857.

Prigent-Combaret, C., Brombacher, E., Vidal, O., Ambert, A., Lejeune, P., Landini, P., & Dorel, C. (2001). Complex regulatory network controls initial adhesion and biofilm formation in Escherichia coli via regulation of thecsgD gene. Journal of bacteriology, 183(24), 7213-7223.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]