Coding
Hin

Part:BBa_J31000:Design

Designed by: Erin Zwack, Sabriya Rosemond   Group: iGEM06_Davidson   (2006-06-05)
Revision as of 20:15, 26 July 2006 by Fizzle6821 (Talk | contribs) (→‎Biobricks)


DNA-invertase Hin from Salmonella typhimurium


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is cloned into plasmid pSB1A2.

Biobricks

The Biobricks attached to this part are not correct.

GAATTCGCGGCCGCTTCTAGA-ATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTACCAGTGCAAATTGTGACCGCATTTTTGAGGACCGTATCAGTGGCAAGATTGCAAACCGCCCCGGCCTGAAACGAGCGTTAAAGTATGTAAATAAAGGCGATACTCTTGTCGTCTGGAAATTAGACAGACTGGGCCGCAGCGTGAAAAACCTGGTGGCGTTAATATCAGAATTACATGAACGTGGAGCTCACTTCCATTCTTTAACCGATAGTATTGATACCAGTAGCGCGATGGGGCGATTCTTTTTTCATGTAATGTCAGCACTGGCCGAGATGGAGCGAGAATTAATTGTCGAGCGAACCCTTGCCGGACTGGCTGCCGCCAGAGCGCAAGGACGACTGGGAGGGCGCCCTCGGGCGATCAACAAACATGAACAGGAACAGATTAGTCGGCTATTAGAGAAAGGCCATCCTCGGCAGCAACTAGCTATTATTTTTGGTATTGGCGTATCTACCTTATACAGATATTTTCCGGCAAGCCGTATAAAAAAACGAATGAATTAA-ACTAGT-GCGGCCGCCTGCAG

There is no G spacer between the XbaI and the insert.

There is no T spacer between the insert and the SpeI site.

There is no A spacer between the SpeI site and the NotI site.

The last C of the NotI site is not conserved with the initial C from the PstI site.

The BB suffix currently has this sequence for NotI and PstI: GCGGCCGcCTGCAG

But it should have been: GCGGCCGCTGCAG

There is no spacer between the NotI site and the SpeI site. There should be a T.

Source

Salmonella typhimurium

References