Part:BBa_K1020008

alkane producing module

This part is composed of genes encoding two enzymes,NPDC and AAR. Both are key enzymes in the production of fungible hydrocarbon biofuels, for they play important roles in turning fatty acyl-ACPs into alkanes.Different from BBa_K590025 given by Washington iGEM 2010, we have done the coden optimization for ''E.coli''.And the sequence is completely different.

We constructed 3 strains to characterize the productivity of intracellular alkane: strain1:No.53：producing module (constitutive promoter J23100)+ Alk-Sensor device strain2:No.54：producing module (constitutive promoter J23114) + Alk-Sensor device strain3:No.55：Null+ Alk-Sensor device The promoter strength of No.54 is stronger than that of No.53. Theorectically, alkane productivity of No.53 and No.54 will be different, and intracellular alkane concentration of the above 3 strains will be different-- No.54 the highest, No.53 lower and No.55 the lowest.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 48
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]