Part:BBa_K1106004:Experience
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Applications of BBa_K1106004
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UNIQ012f1bc04cb556e1-partinfo-00000000-QINU UNIQ012f1bc04cb556e1-partinfo-00000001-QINU
Objective Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concrentrations.
Methods Several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).
Results As shown in the graphics below GFP production increases with the arsenite concentration.
Conclusion The promoter is sensitive to different arsenite concentrations.
