Generator

Part:BBa_K1100009

Designed by: YingQi Lou   Group: iGEM13_Fudan   (2013-09-13)
Revision as of 19:05, 27 September 2013 by Cheese7 (Talk | contribs)

Csy4 generator with no terminator

This part is a intermediate.A seious of parts are put together onto the pSB1C3 plasmid including the promter(BBa_R0040),csy4 insulator(16-nt), a RBS(BBa_B0034) and the Csy4 gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 164


[edit]
Categories
Parameters
biology
controlaTc, tetracyline
directionForward
efficiency1
negative_regulators1
o_h
o_l
positive_regulators
proteinCsy4