Device

Part:BBa_K1051801

Designed by: Shihong Chen   Group: iGEM13_Shenzhen_BGIC_ATCG   (2013-09-08)
Revision as of 18:49, 27 September 2013 by KLBF CSH (Talk | contribs)

Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib

This part consists of promoter SNR52, sgRNA coding sequence and SUP4 terminator. Structure of sgRNA is like the picture below Structure_of_sgRNA.png
sgRNA can help dCas9(BBa_k1051800) to target to hubi to inhibit the expression of hubi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 134
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aTargeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib