Coding
dCas 9

Part:BBa_K1051800

Designed by: Shihong Chen   Group: iGEM13_Shenzhen_BGIC_ATCG   (2013-07-11)
Revision as of 18:31, 27 September 2013 by KLBF CSH (Talk | contribs)

protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon. 

     Protein dCas9 is the key of the technology CRISPRi,and this part is the gene

of the dcas9. Deleting its ending codon is to provide a convenient way to add other
protein domain to improve the function of dCas9. 

      CRISPRi_principle.png
      So this part is used with sgRNA(k1051801,k1051802).

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2740
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2886
    Illegal BsaI site found at 3740
    Illegal BsaI.rc site found at 1411


[edit]
Categories
Parameters
None