Composite

Part:BBa_K1030008

Designed by: Wei Huang   Group: iGEM13_SUSTC-Shenzhen-A   (2013-09-16)
Revision as of 03:18, 17 September 2013 by Registry (Talk | contribs)

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Zeo-resistant gene + GFPuv

We use a zeocin resistant gene and external zeocin to tune the cell growth rate. The common antibiotic resistant genes are reserved for plasmid backbones. GFPuv is used to monitor the expression of zeocin resistant gene. In addition, zeocin resistant gene allosterically inhibitor the effect of zeocin, makes it easy to be quantified and mathematically modeled.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


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Parameters
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