Regulatory

Part:BBa_K1051302

Designed by: Kequan Lin   Group: iGEM13_Shenzhen_BGIC_ATCG   (2013-06-30)
Revision as of 16:47, 27 September 2013 by WHUyoyo (Talk | contribs)

clb5 promoter (during G1 phase)

Purpose

Works to activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p

Principle

It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the sequence which is actived by clb5 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation .it most abundant during late G1 phase; CLB5 has a paralog, CLB6, that arose from the whole genome duplication

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 249
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/a clb5 promoter (during G1 phase)