Composite
Part:BBa_K1149052
Designed by: Margarita Kopniczky Group: iGEM13_Imperial_College (2013-09-26)
Constitutive phaCAB
This part has a strong E.coli constitutive promoter in front of the phaCAB PHB biosynthetic operon.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 627
Illegal BglII site found at 1452 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 604
Illegal NgoMIV site found at 916
Illegal NgoMIV site found at 1195
Illegal NgoMIV site found at 1847
Illegal NgoMIV site found at 1869
Illegal NgoMIV site found at 2491
Illegal NgoMIV site found at 2632 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3713
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Categories
Parameters
None |