Part:BBa_K1031804
Pu-B0032-sfGFP-Terminator (XylR)
Structure
Fig 1
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 167
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 218
Construction and tunning
Pc promoter J23114 is selected to initiate the transcription of XylR. Based on this circuit, we constructed a library of RBS (Ribosome Binding Site) including B0031[1], B0032[2], B0033[3] and B0034[4] for tunning for expression level of reporter gene sfGFP. K1031804 consists of Pu promoter, RBS B0032 and reporter gene sfGFP (Fig 2a). Induction ratio was calculated from fluorescence intensity, showing the performance of K1031804(Fig 2b)
Fig 2 Construction and tunning of reporter circuit Pu-B0032-sfGFP (a) The orange arrow represents Pu promoter for XylR. The green oval stands for RBS B0032. sfGFP coding sequence is shown with dark blue, while terminator B0015[5] is in dark red. (b) Horizontal axis represents biosensor circuit J23114-XylR adopting different RBS. Vertical axis stands for induction ratio. The dashed box refers to data for reporter circuit Pu-B0032-sfGFP in collocation with biosensor circuit J23114-XylR.
//function/reporter/fluorescence
//rbs/prokaryote/constitutive
//terminator/double
device_type | |
n/a | Pu-B0032-sfGFP-Terminator (XylR) |