Part:BBa_K1104100
mngB - alpha-mannosidase
mngB, a passage of gene in Escherichia coli K-12 substr MG1655, can produce alpha-mannosidase. It is an enzyme that turns 2-O-(6-phospho-α-mannosyl)-D-glycerate into mannose-6-phosphate and glycerate, which is the reverse reaction of the post-translation (glycosylation) on polar filaments of Fungi. This degradation pathway is often seen in many organisms, and the reason why we choose from MG1655 is that this species of E-coli are able to live in bees' midgut. Besides MG1655 is also the bacteria that we are going to transform our whole circuit into. This decision make our experiment more easier and more certain that this enzyme can well perform in our Bee. coli. . We tent to overexpress this gene in order to inhibit the production of the PTP1 in the polar filament of N. ceranae, the chosen target fungi that we are eager to eliminate.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 716
Illegal BglII site found at 992
Illegal BglII site found at 2139 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 235
Illegal AgeI site found at 2242 - 1000COMPATIBLE WITH RFC[1000]
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