Device
bluelight
Part:BBa_K1065311:Design
Designed by: Thomas Perli Group: iGEM13_UNITN-Trento (2013-09-18)
Blue-light photoinducible AmilCP + EFE producing device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4114
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 605
Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 767
Illegal NgoMIV site found at 785
Illegal NgoMIV site found at 1297
Illegal NgoMIV site found at 1590
Illegal NgoMIV site found at 1684
Illegal AgeI site found at 319
Illegal AgeI site found at 1465
Illegal AgeI site found at 4865 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1354
Illegal BsaI.rc site found at 218
Design Notes
We designed the device with a reporter gene so the colonies can be screened in an easily way. Moreover, EFE gene is compatible with the RFC25 assembly (Freiburg Assembly) allowing us to fuse that protein with another protein domain.
Source
The device is composed by two subparts created by the UNITN-Trento 2013 iGEM team.