Device
bluelight

Part:BBa_K1065311:Design

Designed by: Thomas Perli   Group: iGEM13_UNITN-Trento   (2013-09-18)
Revision as of 11:36, 18 September 2013 by Registry (Talk | contribs)

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Blue-light photoinducible AmilCP + EFE producing device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4114
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
    Illegal AgeI site found at 4865
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


Design Notes

We designed the device with a reporter gene so the colonies can be screened in an easily way. Moreover, EFE gene is compatible with the RFC25 assembly (Freiburg Assembly) allowing us to fuse that protein with another protein domain.


Source

The device is composed by two subparts created by the UNITN-Trento 2013 iGEM team.

References