Coding
lFTN

Part:BBa_K1189024

Designed by: Chris Wintersinger, Denny Hoang   Group: iGEM13_Calgary   (2013-09-17)
Revision as of 23:44, 24 September 2013 by Dwoppers (Talk | contribs)

Light chain human ferritin

Light ferritin, along with heavy ferritin (hFTN INCLUDE LINKNKN), forms ferritin, a ubiquitous iron-storage nanoparticle. The formed nanoparticle is highly robust, remaining stable at extreme conditions, such as pHs of ______, temperatures of ____, and urea concentrations of _____.

This protein can serve two functions, it can serve as a molecular scaffold, a base upon which proteins can be anchored to. When apart of fusions, ferritin has previously been found to stabilize proteins such as GFP, enhancing the effects of the fused protein. something scaffodl

<includ link for ferritin>

Ferritin has also been used as a reporter system, in a manner similar to horseradish peroxidase. This is done through a reaction known as the Fenton reaction, wherein hydrogen peroxide is homolytically cleaved to produce hydroxide radicals, which then react with certain substrates, such as TMB and ABTS, to produce a colourimetric change. <pictures or something>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 391


[edit]
Categories
Parameters
None