Part:BBa_K1074007
Pctc+RBS+amilCP
Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. Activated by endogenous sigma factor B under mild stress( nutrient stress response or physical stress response). amilCP(BBa_K592009), blue chromoprotein,naturally exhibits strong color when expressed. when bacterial grow to the post-log phase, nutrient and space stress arise,Promoter ctc should be activited. We assemble this gene circuit just to expect a visual signal when B.subtilis grow to the post-log phase.
Usage and Biology
In our experiment, we failed to see obviously blue during all of the grow phases. We have tried almost all of the
Reference
Benson, A.K. and W.G. Haldenwang, Characterization of a Regulatory Network That Controls Sigma-B Expression in Bacillus-Subtilis. Journal of Bacteriology, 1992. 174(3): p. 749-757.
Boylan, S.A., et al., Activation of Bacillus-Subtilis Transcription Factor Sigma(B) by a Regulatory Regulatory Pathway Responsive to Stationary-Phase Signals. Journal of Bacteriology, 1992. 174(11): p. 3695-3706.
Boylan, S.A., et al., Stress-Induced Activation of the Sigma(B) Transcription Factor of Bacillus-Subtilis. Journal of Bacteriology, 1993. 175(24): p. 7931-7937.
Brody, M.S., K. Vijay, and C.W. Price, Catalytic function of an alpha/beta hydrolase is required for energy stress activation of the sigma(B) transcription factor in Bacillus subtilis. Journal of Bacteriology, 2001. 183(21): p. 6422-6428.
Delumeau, O., R.J. Lewis, and M.D. Yudkin, Protein-protein interactions that regulate the energy stress activation of sigma(B) in Bacillus subtilis. Journal of Bacteriology, 2002. 184(20): p. 5583-5589.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 78
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 78
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 78
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 78
- 1000COMPATIBLE WITH RFC[1000]
| None |