Composite

Part:BBa_K1017811

Designed by: Wen-Hui Cheng   Group: iGEM13_NCTU_Formosa   (2013-09-12)
Revision as of 16:38, 26 September 2013 by VivianLin (Talk | contribs)

Pcons+rRBS-2+mRFP+J61048

Pcons is a constitutive promoter which can be used to turn the expression level. rRBS-2 is a sRNA binding site that is designed by iGEM13_NCTU_Formosa. When the sRNA from K1017402 bind on it, this part will be shut down. Then, the downstream gene mRFP won't be made. mRFP(E1010) is a red fluorescent protein that functions as a reporter. We made this biobrick in order to test whether our self-designed rRBS-2 really works. Also, we want to compare this rRBS-2’s strength with other RBS(B0030, B0032, B0034.)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 784
    Illegal AgeI site found at 625
    Illegal AgeI site found at 737
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None