Composite

Part:BBa_K1017811

Designed by: Wen-Hui Cheng   Group: iGEM13_NCTU_Formosa   (2013-09-12)
Revision as of 15:41, 25 September 2013 by H89161h (Talk | contribs)

Pcons+rRBS-2+mRFP+J61048

Pcons is a constitutive promoter family member(from J23100 to J23119) which can be used to turn the expression level of express part and we choose J23101. rRBS-2 is a sRNA binding site that is designed by iGEM13_NCTU_Formosa. When the sRNA from K1017402 bind on it, this part will be shut down. Then, the gene downstream won't be made. mRFP(E1010) is a red fluorescent protein that functions as a reporter. J61048 is a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 784
    Illegal AgeI site found at 625
    Illegal AgeI site found at 737
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None