Protein_Domain
MLS

Part:BBa_K1119001:Design

Designed by: Wai In Hui   Group: iGEM13_Hong_Kong_HKUST   (2013-09-11)
Revision as of 15:01, 26 September 2013 by Klchuab (Talk | contribs)

Mitochondrial Leader Sequence with RFC25 standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in RFC25 standard, which facilitate protein fusion in order to introduce other protein into mitochondria.


Source

The CDS of MLS was cloned out from pCMV/myc/mito (Invitrogen, Carlsbard, CA) using PCR with primers that includes prefix and suffix in RFC25 standard. The original source of this CDS of MLS sequence is from that of subunit VIII of human cytochrome c oxidase. (Invitrogen, Carlsbard, CA)

References

pCMV myc/mito(Invitrogen™) http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf Dean, J., Tran , L., Liao, J., Dipple, K., Reue, K., Tontonoz, P., & Beaven, S. (2009). Resistance to diet-induced obesity in mice with synthetic glyoxylate shunt. Cell Metabolism, 9((6)), 525–536. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/19490907