Regulatory
pLas + RBS

Part:BBa_K1073003

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-16)
Revision as of 13:39, 26 September 2013 by Laura S (Talk | contribs)

Inducible promoter of the las quorum sensing system with RBS

This construct is derived from BBa_K091117 and BBa_B0032. It combines the inducible promoter of th las Quorum sensing system of Pseudomonas aeruginosa which is activated by LasR in combination with N-oxododecanoyl homoserine lactone (N-oxododecanoyl-HSL) with a RBS.


Usage and Biology

The inducible promoter from Pseudomonas aeruginosa is activated by LasR complexed with the specific autoinducer N-oxododecanoyl-HSL produced by LasI. The induction of the promoter in P. aeruginosa leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003]. The device can be cloned in front of a desired gene of interest in order to induce its Expression by N-oxododecanoyl-HSL. For further Information see BBa_R0079.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None