Composite

Part:BBa_K1139022

Designed by: Naoki Watarai   Group: iGEM13_Tokyo_Tech   (2013-09-24)
Revision as of 08:39, 24 September 2013 by Registry (Talk | contribs)

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Promoterless-M13-Plac-GFP on pSB3

M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell. This biobrick is derived from M13mp18 phage vector, amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and Lac promoter. The promoter on the upstream of g2(gene 2) is deleted.The capsid is formed only when g2p(gene 2 protein) is expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 100
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6026
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7287


[edit]
Categories
Parameters
n/aPromoterless-M13-Plac-GFP