Protein_Domain
MLS
Part:BBa_K1119001
Designed by: Wai In Hui Group: iGEM13_Hong_Kong_HKUST (2013-09-11)
Mitochondrial Leader Sequence with RFC25 standard
Mitochondrial leader sequence (MLS) helps direct protein to the mitochondria. When this sequence is cloned in frame at the N-terminus of the protein, it can target the recombinant protein to the mitochondria in mammalian cells. MLS will be removed upon translocation into the mitochondrial matrix. However, at least four additional amino acids (Ile-His-Ser-Leu) on this sequence are known to be retained on the N-terminus of the protein.
Since MLS always fuses with protein for function, this BioBrick is in RFC 25 standard, which facilitate protein fusion in order to introduce other protein into mitochondria.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/eukaryote/human
//proteindomain/localization
//proteindomain/localization
None |