Regulatory
Prm/lac

Part:BBa_K1139151

Designed by: Naoki Watarai   Group: iGEM13_Tokyo_Tech   (2013-09-24)
Revision as of 03:29, 26 September 2013 by Keso57 (Talk | contribs)

rm/lac hybrid promoter

1. Introduction
We newly developed rm/lac hybrid promoter which is activated by CI and repressed by LacI (Fig. 1-A).
To characterize the function of this hybrid promoter, we constructed a part Prm/lac-GFP (BBa_K1139150).

Fig. 1-A. Our new designed hybrid promoter

To characterize the function of the rm/lac hybrid promoter (BBa_K1139151), we constructed this part Prm/lac-GFP (BBa_K1139150) by inserting rm/lac promoter in front of a GFP coding sequence.
By using the reporter cell that contains Prm/lac-GFP, we measured the fluorescence intensity of the reporter cell dependent on the four different combinations of two inducers, CI and IPTG (Fig. 1-B).

Fig. 1-B. Comparison of N99 and JM2.300


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/arm/lac hybrid promoter