Composite

Part:BBa_K1140006:Design

Designed by: Janssel Reyes del Castillo   Group: iGEM13_UANL_Mty-Mexico   (2013-09-13)
Revision as of 21:04, 25 September 2013 by Quintero (Talk | contribs) (→‎Source)

pTet + 37 oC RNA thermometer + mCherry (LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 63
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 676
    Illegal AgeI site found at 788
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.

Source

Obtained synthetically. The RNA thermosensor sequence was obtained and adapted from [1] (u6 synthetic RNA thermometer). The mCherry coding sequence was optimized for E. coli codon usage. The pTet promoter is identical to the sequence found in part BBa_R0040.

References

Elowitz, MB and Leibler, S, (2000). A synthetic oscillatory network of transcriptional regulators, Nature, 20;403(6767):335-8.

Neupert J, Karcher D, Bock R (2008) Design of simple synthetic RNA thermometers for temperature- controlled gene expression in Escherichia coli. "Nucleic Acids Res, 36(19):e124.