Device
Part:BBa_K1065309
Designed by: Fabio Digiacomo Group: iGEM13_UNITN-Trento (2013-09-18)
light regulated circuit producing amilGFP+EFE at dark
This part includes blue light sensor YF1, its RR fixJ,PfixK2,the reporter amilGFP and EFE+terminators: everything is under the regulation of Plac. under blue light fixJ is phosphorilated and inhibits the activity of Pfixk2 consisting in production of amilGFP+EFE only at dark.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3309
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 770
Illegal NgoMIV site found at 842
Illegal NgoMIV site found at 932
Illegal NgoMIV site found at 950
Illegal NgoMIV site found at 1462
Illegal NgoMIV site found at 1755
Illegal NgoMIV site found at 1849
Illegal AgeI site found at 484
Illegal AgeI site found at 1630
Illegal AgeI site found at 4060 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1519
Illegal BsaI.rc site found at 383
[edit]
Categories
Parameters
None |