Device
Rubber Fuc

Part:BBa_K1088024

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-09-18)
Revision as of 11:38, 18 September 2013 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


HRT2 prenyltransferase from Hevea Brasilianis (ara promoter without araC: arabinose inducible)

This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber.

The gene is expressed from the arabinose promoter (BBa_I13453), which is induceable with arabinose under glucose scarce conditions. Note that the regulater insn't a part of this part and thus no/very little transcription is expected.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None