Composite

Part:BBa_K1140006

Designed by: Janssel Reyes del Castillo   Group: iGEM13_UANL_Mty-Mexico   (2013-09-13)
Revision as of 01:33, 19 September 2013 by GilbertoRdz (Talk | contribs)

pTet + 37 oC RNA thermometer + mCherry (LVA)

Codifies for a RFP with a LVA tag. The RFP is under the regulation of a pTet promoter and a thermolabile ribosome binding site with an optimal translation temperature of 37 °C. Two transcription termination sites were also added at the 3’ end of the gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 63
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 676
    Illegal AgeI site found at 788
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//function/reporter/fluorescence
Parameters
directionForward
functionIntegrated Transcriptional and Translational regulation
proteinmCherry
switch_point37 celsius degrees
tagLVA