Composite

Part:BBa_K1073034

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 13:38, 17 September 2013 by Registry (Talk | contribs)

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Inducible ampR expression combined with LasR, RhlI and aeBlue expression cassettes

This construct is the basis for a Quorum sensing system for two bacteria. The selection marker (amp-resistance) is induced by LasI(inducer)/LasR(activator) complex. The LasR is encoded on the construct itself, whereas the LasI is taken up from the media. Furthermore the RhlI synthetase is expressed to synthesize the autoinducer RhlI for the corresponding system in another cell (BBa_K1073035).

By the activation of the amp-resistance the gene of interest (in this case the chromoprotein aeblue) is expressed. Together with BBa_K1073035 the construct forms a dependency between two cells (one with BBa_K10733034, the other with BBa_K1073035).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1306
    Illegal NheI site found at 1329
    Illegal NheI site found at 2988
    Illegal NheI site found at 3011
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2830
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1683
    Illegal AgeI site found at 1880
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None