Regulatory

Part:BBa_K1045000

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-06-24)
Revision as of 22:41, 24 September 2013 by Kati (Talk | contribs)

DarR operator

This part is the binding sequence of the c-di-AMP-dependent regulatory protein DarR. When bound by DarR, it is supposed to prevent the downstream information to be transcribed. The important information: The operator is not in the pSBC13 Vector but instead in the pBluescript -KS plasmid. This plasmid provides an Ampicillin resistance. Furthermore it has got a pUC origin.

(Reference: Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aDarR operator